IntroductionĬaffeine is one of the most commonly consumed drugs, with a long history of usage, being commonly used to improve mental alertness and alleviate fatigue. These data suggest that the method holds promise for the determination of caffeine in such samples. Good agreement was also obtained with the stated values of caffeine for an energy drink and for Coca-Cola®. Replicate caffeine determinations on fortified latte style white coffee and Pepsi Max® gave mean recoveries of 93.4% (%CV = 4.1%) and 95.0% (%CV = 0.98%), respectively. No interferences were recorded from endogenous beverage components or from commonly occurring drugs, such as nicotine, ibuprofen, and paracetamol. The theoretical limit of detection (3 σ) was 0.456 μg/mL. The response was linear over the range 10 to 500 μg/mL ( R 2 = 0.996). A 5 μL aliquot was introduced to the thermal desorption unit, dried, and thermally desorbed to the GC/MS. Using a pyrolysis-gas chromatography mass spectrometry system, the conditions required for the direct thermal desorption-gas chromatography mass spectrometry (TD-GC/MS) determination of caffeine were optimised. An extraction-free method requiring microliter ( μL) volumes has been developed for the determination of caffeine in beverages.
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